LOCUS       40924_27104        7327 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pKU1021ggs DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Yamada Y, Kuzuyama T, Komatsu M, Shin-Ya K, Omura S, Cane DE, Ikeda 
            H.
  TITLE     Terpene synthases are widely distributed in bacteria
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 112 (3), 857-862 (2015)
  PUBMED    25535391
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Ikeda H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2014) Contact:Haruo Ikeda Kitasato University, 
            Kitasato Institute for Life Sciences; 1-15-1 Kitasato, Sagamihara, 
            Kanagawa 252-0373, Japan URL :http://avermitilis.ls.kitasato-u.ac.jp
REFERENCE   3  (bases 1 to 7327)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7327)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2015"; volume: "112"; issue: "3"; pages: 
            "857-862"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-2014) Contact:Haruo Ikeda Kitasato University, Kitasato 
            Institute for Life Sciences"; volume: " 1-15-1 Kitasato, Sagamihara,
            Kanagawa 252-0373, Japan URL :http"; pages: 
            "//avermitilis.ls.kitasato-u.ac.j"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             353..1144
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     oriT            complement(1485..1594)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1648..1659)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     protein_bind    1730..1829
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     CDS             1839..3653
                     /label=phage phi-C31 integrase
                     /note="integrase from phage phi-C31"
     rep_origin      3876..4464
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4752..4773
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4788..4818
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4826..4842
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4850..4866
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4910..5995)
                     /codon_start=1
                     /gene="ggs"
                     /product="geranylgeranyl diphosphate synthase"
                     /label=ggs
                     /note="from Streptomyces sp."
                     /protein_id="BAP82241.1"
                     /translation="MAGTAWDTDIPTLISVRDDVDAVLEDFVDVQERAAHGPEMAPLFT
                     TARRLLFSNGKRLRPLLCVAGWQAAGAPGDPHAVLRVAAALELFQTFALIHDDVMDDSD
                     TRRGRPSAHRALAAEYMSGGGRLSKAEAHGRGAAILLGDLLLVWSDEMLAGAGLEARAR
                     ARVLPLVDSMRAELVYGQYLDLLSTDRMSGDVEAALRVARYKTGKYTVERPLHLGVSLA
                     GGDPRLLQTCTDFALPLGEAFQLRDDLLNVFGDPVRTGKPVGDDIREGKATVLLALAIQ
                     DASEADLKLLGTLVGSPELGSEDIDRFRTVMETTGARQRVEDMIIARRDTALATLEEAD
                     LPQDVAETLRQIVWMATERQS"
     gene            complement(4910..5995)
                     /gene="ggs"
                     /label=ggs
     regulatory      complement(5999..6545)
                     /gene="rpsJp"
                     /note="promoter of gene encoding ribosomal protein S10;
                     from Streptomyces avermitilis MA-4680"
                     /regulatory_class="promoter"
     gene            complement(5999..6545)
                     /gene="rpsJp"
                     /label=rpsJp
     promoter        6550..6578
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             6767..7297
                     /label=GmR
                     /note="gentamycin acetyltransferase"
ORIGIN
        1 ttcacgctgc cgcaagcact cagggcgcaa gggctgctaa aggaagcgga acacgtagaa
       61 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac
      121 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata
      181 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
      241 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg
      301 atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
      361 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
      421 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
      481 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tccaagacga
      541 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
      601 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
      661 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
      721 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
      781 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
      841 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cggatgcccg acggcgagga
      901 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
      961 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     1021 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     1081 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     1141 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     1201 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     1261 gacgccctcg cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac
     1321 ggccagcagg taggccgaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct
     1381 tcgttcgtct ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt
     1441 ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag
     1501 agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc
     1561 gctcgcgggt gggcctactt cacctatcct gccccgctga cgccgttctc gagcgtgccg
     1621 gagcaatcgc cctgggtggg ttacacgacg cccctctatg gcccgtactg acggacacac
     1681 cgaagccccg gcggcaaccc tcagcggatg ccccggggct tcacgttttc ccaggtcaga
     1741 agcggttttc gggagtagtg ccccaactgg ggtaaccttt gagttctctc agttgggggc
     1801 gtagggtcgc cgacatgaca caaggggttg tgaccggggt ggacacgtac gcgggtgctt
     1861 acgaccgtca gtcgcgcgag cgcgagaact cgagcgcagc aagcccagcg acacagcgta
     1921 gcgccaacga agacaaggcg gccgaccttc agcgcgaagt cgagcgcgac gggggccggt
     1981 tcaggttcgt cgggcatttc agcgaagcgc cgggcacgtc ggcgttcggg acggcggagc
     2041 gcccggagtt cgaacgcatc ctgaacgaat gccgcgccgg gcggctcaac atgatcattg
     2101 tctatgacgt gtcgcgcttc tcgcgcctga aggtcatgga cgcgattccg attgtctcgg
     2161 aattgctcgc cctgggcgtg acgattgttt ccactcagga aggcgtcttc cggcagggaa
     2221 acgtcatgga cctgattcac ctgattatgc ggctcgacgc gtcgcacaaa gaatcttcgc
     2281 tgaagtcggc gaagattctc gacacgaaga accttcagcg cgaattgggc gggtacgtcg
     2341 gcgggaaggc gccttacggc ttcgagcttg tttcggagac gaaggagatc acgcgcaacg
     2401 gccgaatggt caatgtcgtc atcaacaagc tcgcgcactc gaccactccc cttaccggac
     2461 ccttcgagtt cgagcccgac gtaatccggt ggtggtggcg tgagatcaag acgcacaaac
     2521 accttccctt caagccgggc agtcaagccg ccattcaccc gggcagcatc acggggcttt
     2581 gtaagcgcat ggacgctgac gccgtgccga cccggggcga gacgattggg aagaagaccg
     2641 cttcaagcgc ctgggacccg gcaaccgtta tgcgaatcct tcgggacccg cgtattgcgg
     2701 gcttcgccgc tgaggtgatc tacaagaaga agccggacgg cacgccgacc acgaagattg
     2761 agggttaccg cattcagcgc gacccgatca cgctccggcc ggtcgagctt gattgcggac
     2821 cgatcatcga gcccgctgag tggtatgagc ttcaggcgtg gttggacggc agggggcgcg
     2881 gcaaggggct ttcccggggg caagccattc tgtccgccat ggacaagctg tactgcgagt
     2941 gtggcgccgt catgacttcg aagcgcgggg aagaatcgat caaggactct taccgctgcc
     3001 gtcgccggaa ggtggtcgac ccgtccgcac ctgggcagca cgaaggcacg tgcaacgtca
     3061 gcatggcggc actcgacaag ttcgttgcgg aacgcatctt caacaagatc aggcacgccg
     3121 aaggcgacga agagacgttg gcgcttctgt gggaagccgc ccgacgcttc ggcaagctca
     3181 ctgaggcgcc tgagaagagc ggcgaacggg cgaaccttgt tgcggagcgc gccgacgccc
     3241 tgaacgccct tgaagagctg tacgaagacc gcgcggcagg cgcgtacgac ggacccgttg
     3301 gcaggaagca cttccggaag caacaggcag cgctgacgct ccggcagcaa ggggcggaag
     3361 agcggcttgc cgaacttgaa gccgccgaag ccccgaagct cccccttgac caatggttcc
     3421 ccgaagacgc cgacgctgac ccgaccggcc ctaagtcgtg gtgggggcgc gcgtcagtag
     3481 acgacaagcg cgtgttcgtc gggctcttcg tagacaagat cgttgtcacg aagtcgacta
     3541 cgggcagggg gcagggaacg cccatcgaga agcgcgcttc gatcacgtgg gcgaagccgc
     3601 cgaccgacga cgacgaagac gacgcccagg acggcacgga agacgtagcg gcgtagcgag
     3661 acacccggga agcctgttag gcgctgagac gggcgcacag cgggcttcct ggggcaatgc
     3721 atcctccagc gcggggatct catgctggag ttcttcgccc accccaaaag gatctaggtg
     3781 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     3841 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3901 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3961 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4021 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4081 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4141 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4201 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4261 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4321 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4381 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4441 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4501 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4561 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     4621 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     4681 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     4741 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     4801 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     4861 tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg actctagact catgactgcc
     4921 tttccgtggc catccatacg atctgcctca acgtctcggc gacgtcctgg ggcaggtctg
     4981 cttcttccag cgtggcgagg gcggtgtcgc gcctggcgat gatcatgtct tcgacccgct
     5041 gccgggcacc ggtggtctcc atgacggtgc ggaaccggtc gatgtcctcg ctccccagtt
     5101 ccgggctgcc gaccagggtg ccgagcagct tcaggtcggc ctcggaggcg tcctggatcg
     5161 cgagagccag gagcacggtg gctttgccct cacggatgtc gtctccgaca ggcttgccgg
     5221 tccgcaccgg gtcaccgaag acgttgagca ggtcatcgcg cagctgaaac gcttctccca
     5281 gcgggagggc gaaatcggtg caggtctgca gcagccgcgg atcgccgccg gccagggaaa
     5341 cgccgaggtg caggggccgt tccacggtgt acttccccgt cttgtaccgg gcgacccgca
     5401 gcgcggcctc gacgtccccg gacatccggt cggtcgacag cagatccagg tactgcccgt
     5461 agaccagctc ggcgcgcatg ctgtccacca gtggcaggac cctggcccgc gcccgggctt
     5521 cgagccccgc gcccgccagc atttcgtccg accacacgag caggaggtca ccgaggagga
     5581 tggcggcacc tcggccgtgc gcctccgcct tgctgagccg cccaccgccg ctcatgtact
     5641 cggcggccag cgcgcggtgg gccgagggcc gtccgcggcg ggtgtcactg tcgtccatga
     5701 cgtcgtcgtg gatcagggcg aaggtctgga acagctccag ggcggcggcg acgcgaagga
     5761 ccgcgtgggg gtctcccggt gcgcctgcgg cctgccatcc cgccacgcac agcaaggggc
     5821 gcagtcgctt gccgttggag aagaggagcc ggcgggccgt ggtgaacagc ggtgccattt
     5881 ccgggccgtg cgctgcccgc tcctgcacgt cgacgaagtc ctcgagcacc gcgtctacat
     5941 cgtcacgaac ggatataagg gtcgggatat cggtgtccca tgcggtaccg gccatatgta
     6001 ctcagtagtc cttcgtctcg ataaaacgct ctggagcccg ggggttcgcg tcctgacctc
     6061 cgacccacgc ggtcgggtgt gtcgcgctct tgctgacaga gatatccgat atggatttcc
     6121 ctaccgaggg acacggcccg aaaaaaaccg caggaccggg ggcacaacac ccaccgggcg
     6181 cctggtcggc accccgctga aacttcccgg aagattcccg tacgtccgcc ccagcgctgc
     6241 ctttcgacag atcgggcgac gagtactgtg ggactcgctt ccagtcctcc cggcgggagg
     6301 cgcacagcat cggcactcga ccgagcaact ccgacagtct gccatacggg gcacgggcct
     6361 ggccaatcgg gccgaggatc gtaccccgag ggtgacgcag gtcaaacccc gggcactcag
     6421 caagggtcgc acaccgatcc gcccggggca cacgcgtccg accccggtgc caccggccgg
     6481 ccgggaacgt gagcacatgt gctcatcccg gcagccccct tccgggtgaa cagaacatgt
     6541 ccgtggaatt ggacataagc ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc
     6601 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     6661 ttcatggctt gttatgactg tttttttgta cagtctatgc ctcgggcatc caagcagcaa
     6721 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     6781 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaggtggct caagtatggg
     6841 catcattcgc acatgtaggc tcggccctga ccaagtcaaa tccatgcggg ctgctcttga
     6901 tcttttcggt cgtgagttcg gagacgtagc cacctactcc caacatcagc cggactccga
     6961 ttacctcggg aacttgctcc gtagtaagac attcatcgcg cttgctgcct tcgaccaaga
     7021 agcggttgtt ggcgctctcg cggcttacgt tctgcccagg tttgagcagc cgcgtagtga
     7081 gatctatatc tatgatctcg cagtctccgg cgagcaccgg aggcagggca ttgccaccgc
     7141 gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt gcttatgtga tctacgtgca
     7201 agcagattac ggtgacgatc ccgcagtggc tctctataca aagttgggca tacgggaaga
     7261 agtgatgcac tttgatatcg acccaagtac cgccacctaa caattcgttc aagccgagat
     7321 cgctagc
//
